CRL-1648 CA46 Burkitts 人淋巴瘤細(xì)胞系，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和最優(yōu)培養(yǎng)條件！


CRL-1648 CA46 Burkitts 人淋巴瘤細(xì)胞系 的詳細(xì)介紹

CRL-1648 CA46 Burkitts 人淋巴瘤細(xì)胞系



ATCC® Number:

CRL-1648™   

Price:

$399.00






Designations:

CA46



Depositors:

IT Magrath



Isotype:

IgM; kappa light chain



Biosafety Level:

1



Shipped:

frozen



Medium & Serum:

See Propagation



Growth Properties:

suspension



Organism:

Homo sapiens (human)



Morphology:

lymphoblast





Source:

Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;



Cellular Products:

immunoglobulin (surface and secreted)



Permits/Forms:

In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.







Receptors:

complement



Antigen Expression:

HLA B27 (the patient was HLA A2, A11, B17, B27)



DNA Profile (STR):

Amelogenin: X
CSF1PO: 10,12
D13S317: 8,12
D16S539: 11,12
D5S818: 13
D7S820: 11,12
THO1: 7,9
TPOX: 8,9
vWA: 15,16



Cytogenetic Analysis:

46, XO, XY, dup1(q21-32), t(8;14) (8pter --> 8q23 :: 14q32 --> 14qter; 14pter --> 14q32 :: 8q23 --> 8qter), 13q+, +16



Gender:

male



Comments:

The cells are EBNA negative and are negative for Fc receptors.
Approximately 12% of the cells express commplement receptors.



Propagation:

ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C



Subculturing:

Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml.
A saturation density of 2 X 10 exp6 is obtainable.



Preservation:

culture medium 95%; DMSO, 5%